Analytical Instrumentation

IE 304 ANALYTICAL INSTRUMENTATION

ﾂ
Objectives:

1. To understand basic principles of various analytical instruments

2. To understand instrumentation required for different types of analytical instruments

3. To know the typical clinical and industrial applications of analytical instrumnets

Unit 1:

Introduction to Chemical instrumental analysis, advantages over classical methods, classification: Spectral, electro analytical and separative methods, Laws of photometry (Beer and Lambert's law), Basic Components of analytical instruments.

[08 Hrs]

Unit 2:

Colourimeters, spectrophotometers (UV-Visible), monochromators, filters, grating, prism, dual wavelength and double monochromator systems, rapid scanning spectrophotometers, IR spectrophotometers.

[08 Hrs]

Unit 3:

Flame Photometry: Principle, constructional details, flue gases, atomizer, burner, optical system, recording system. Atomic absorption spectrophotometers: Theoretical concepts, instrumentation: hollow cathode lamps, burners and flames, plasma excitation sources, optical and electronic system.

[08 Hrs]

Unit 4

Industrial Gas analyzers, pH, conductivity, particle counting, detection on the basis of scattering- Nephalomete, Laboratory Instruments: Centrifuge, oven, waterbath, Incubators, stirrers, Densitometer

[08 Hrs]

Unit 5

Mass Spectrometer (MS): Principle, ionization methods, mass analyzer types - magnetic deflection type, time of flight, quadruple, double focusing, detectors for MS, applications X-ray spectrometry: Instrumentation for X-ray spectrometry, X-ray diffractometer.

[08 Hrs]

Unit 6:

Chromatography: Classification, Gas chromatography: principle, constructional details, GC detectors, Liquid Chromatography, High Performance Liquid Chromatography (HPLC): principle, constructional details, HPLC detectors.

[08 Hrs]

Textbooks:

ﾂ｡ Instrumental Methods of Analysis, Willard, Merritt, Dean, Settle, CBS Publishers & Distributors, New Delhi, Seventh edition.

ﾂ｡ Handbook of Analytical Instruments, R. S. Khandpur, Tata McGraw窶滴ill Publications, 3rd edition

ﾂ｡ Principles of Instrumental Analysis, Skoog, Holler, Nieman, Thomson books-cole publications, 5th edition.

Reference books:

ﾂ｡ Instrumental Methods of Chemical Analysis, Galen W. Ewing, McGraw-Hill Book Company, Fifth edition.

ﾂ｡ Introduction to Instrumental Analysis, Robert D. Braun, McGraw-Hill Book Company.